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pBS-TRE-mTagBFP2-WPRE

Plasmid

#193332

Purpose

Expresses mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[P4-P6(3xPP7)]

Plasmid

#68432

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL3664

Plasmid

#162240

Purpose

Transcribes E. coli SRP RNA sequence for SHAPE-Seq

Depositor

Julius Lucks

Article

Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

Inserts

E. coli SRP RNA
HDV

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3xAla

Plasmid

#233921

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3xAla

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-DHF

Plasmid

#233922

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-DHF

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-1x

Plasmid

#233923

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-1x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3x

Plasmid

#233925

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-5x

Plasmid

#233927

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-5x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS_bDD

Plasmid

#233931

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-bDD

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-11xTA

Plasmid

#233932

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-11xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-15xTA

Plasmid

#233933

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 15 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-15xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-17xTA

Plasmid

#233934

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-17xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-20xTA

Plasmid

#233935

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-20xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-TIR

Plasmid

#233919

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-TIR

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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PMKO-sh_lnc-BATE1

Plasmid

#64866

Purpose

Expresses shRNA targeting lnc-BATE1

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

shRNA targeting lnc-BATE1

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

Plasmid

#163881

Purpose

Acute Depletion of Mau2-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Has Service

AAV1

Insert

hM4D-2a-GFP (CHRM4 Human, Synthetic)

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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