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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 GlyR IRES EGFP

Plasmid

#119739

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CAG

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLVCT-tTR-KRAB

Plasmid

#11643

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Plasmid

#172508

Purpose

Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-EGFP-HaloTag2

Plasmid

#41742

Depositor

Craig Crews

Article

Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

Insert

Halotag 2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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DBDmut-CA-RIT-NFAT1

Plasmid

#63669

Purpose

Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding

Depositor

Anjana Rao

Article

Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

Insert

NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

3x HA

Expression

Mammalian

Mutation

CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
Flag-PML IV/pRK5

Plasmid

#59742

Purpose

Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

PML isoform IV (PML Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP Donor

Plasmid

#31475

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14GFP

Use

Lentiviral

Mutation

Deleted 14 amino acids on the c terminus of eGFP

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-CjCas9

Plasmid

#89752

Purpose

Expression of CjCas9 in mammalian cells

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cas9

Use

CRISPR

Tags

SV40-HA

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPtPuc3

Plasmid

#62863

Purpose

Episome vector for Phaeodactylum tricornutum (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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