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[pSK122] pETDuet GST-p18(delta5)

Plasmid

#136144

Purpose

bacterial expression of GST-p18

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

GST-p18

Tags

GST

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2b_enh_226_Citrine

Plasmid

#130618

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2b_enh_226

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

Plasmid

#70745

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco

Plasmid

#40200

Depositor

Patrick Moore

Article

Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp2x 2A Hygro

Plasmid

#102807

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp2x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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JunD

Plasmid

#187904

Purpose

Expresses JunD in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

JunD (Jund Mouse)

Expression

Mammalian

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-GH023 - NanoLuc

Plasmid

#117727

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDL0_158

Plasmid

#210779

Purpose

PUSCT_Drosophila melanogaster Gypsy-insulator sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Gypsy insulator

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pNeuroD1-ERT2CreERT2

Plasmid

#237402

Purpose

Tamoxifen-dependent expression of ERT2CreERT2 in NeuroD1-positive cells

Depositor

Yusuke Kishi

Article

Sakai et al Development. 2025 Jul 1;152(13):dev20456

Insert

ERT2CreERT2

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS309

Plasmid

#228230

Purpose

GFP dropout backbone for sgRNA library cloning

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pOTIII.EFGP.IGR3.6

Plasmid

#40866

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

MOUSE OXYTOCIN GENE (Oxt Mouse)

Expression

Bacterial

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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RAB14 shRNA

Plasmid

#61496

Purpose

to knockdown endogenous RAB14 expression levels

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag

Plasmid

#224894

Purpose

Mammalian expression of LgBiT-MYPT1(1--314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

LgBiT-MYPT1(1-314)KAKA-Flag

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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