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pAAV-ADP-MCS-FLAG

Plasmid

#192360

Purpose

Adipocyte-specific overexpression of genes

Depositor

Yifu Qiu

Article

Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

Type

Empty backbone

Use

AAV

Tags

3xflag

Expression

Mammalian

Promoter

adiponectin promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-KASH2ext

Plasmid

#187018

Purpose

Control for inducible disruption of LINC Complex

Depositor

Jan Lammerding

Article

Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

Insert

KASH2ext

Tags

GFP

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Gateway Cloning

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDB4283

Plasmid

#98702

Purpose

A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

gRNA PCR template

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Tags

Histag-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRosa26-EF1a-hCas9IRESneo

Plasmid

#67987

Purpose

Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-hCas9IRESneopA

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC42

Plasmid

#66564

Purpose

sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4_TAF15

Plasmid

#26368

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

TAF15 (TAF15 Human)

Use

Gateway Cloning

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pENTR223-BZLF1(Zta)

Plasmid

#195787

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF1 (BZLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRGM_WT

Plasmid

#82134

Purpose

Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRGM (IRGM Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-VRK2

Plasmid

#23534

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK2 (VRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CLK2

Plasmid

#23685

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CLK2 (CLK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_TUBB_WT

Plasmid

#81990

Purpose

Gateway Donor vector containing TUBB, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TUBB (TUBB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.T80K

Plasmid

#81511

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway Cloning

Mutation

T80K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MLST8_WT

Plasmid

#82258

Purpose

Gateway Donor vector containing MLST8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MLST8 (MLST8 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PAK1_WT

Plasmid

#82220

Purpose

Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PAK1 (PAK1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF2_WT

Plasmid

#82204

Purpose

Gateway Donor vector containing ATF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF2 (ATF2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PKDCC

Plasmid

#23387

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKDCC (PKDCC Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

EGFP

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only