162,221 results

1. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209657

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PM-B

   Plasmid

   #209876

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAp63alpha-FLAG

   Plasmid

   #27008

   Depositor

   David Sidransky

   Insert

   TAp63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   dSV40 Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_SARS2_JN.1

   Plasmid

   #218678

   Purpose

   expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (JN.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L455S on the basis of BA.2.86

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP‐RNASEH2B

   Plasmid

   #108697

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2B (RNASEH2B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFD5_w

   Plasmid

   #112645

   Purpose

   Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

   Depositor

   Michael Boutros

   Insert

   U6:3-tRNA-sgRNA expression cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA24N-ligase

   Plasmid

   #87741

   Purpose

   IPTG-inducible expression of T4 DNA ligase for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2TK cyclin T1 (P#468)

   Plasmid

   #14607

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (CCNT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

10. Lifeact-mCherry

    Plasmid

    #193300

    Purpose

    mammalian transient expression - expression of Lifeact-mCherry

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    Lifeact
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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