We narrowed to 8,846 results for: FIE
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Plasmid#97056PurposeExpressing Sulfolobus solfataricus orf SSO1404 proteinDepositorInsertS. solfataricus orf SSO1404
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF239
Plasmid#97025PurposeExpressing Pyrobaculum spherical virus orf239 proteinDepositorInsertPSV orf 239
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF165A
Plasmid#97024PurposeExpressing Pyrobaculum spherical virus orf165a proteinDepositorInsertPSV orf 165a
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF137
Plasmid#97023PurposeExpressing Pyrobaculum spherical virus orf137 proteinDepositorInsertPSV orf 137
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA1376
Plasmid#97002PurposeExpressing MRSA sar1376 proteinDepositorInsertPutative 4-oxalocrotonate tautomerase
TagsN-ter TEV protease cleavable 6HisMutationnonePromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF131
Plasmid#97022PurposeExpressing Pyrobaculum spherical virus orf131 proteinDepositorInsertPSV orf 131
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4511
Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSATAG
Plasmid#97004PurposeExpressing MRSA TAG proteinDepositorInsertDNA-3-methyladenine glycosylase I
TagsN-ter TEV protease cleavable 6HisPromoterT7Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA4
Plasmid#89722PurposeKnockout IRF8 in human cellsDepositorAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA3
Plasmid#89721PurposeKnockout IRF8 in human cellsDepositorAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-TP1107(Q15TAG)
Plasmid#247462PurposeBacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporationDepositorInsertTP1107 Q15TAG
TagsHis6 and TEV protease cleavage siteExpressionBacterialMutationOriginal Q15 codon (CAA) was modified to amber st…PromoterT7Available SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable SinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmSTING
Plasmid#208386PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.DepositorAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128589PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterSynAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only