176,531 results
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pAAV-CAG-mRuby2
Plasmid#99123PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
TagsmCherryExpressionMammalianPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABEpuro mTurquoise2 LC3B
Plasmid#78518PurposeFluorescent reporter for LC3B visualizationDepositorInsertLC3B (MAP1LC3B Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianPromoterretroviralAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-gfap
Plasmid#82401PurposeGateway-compatible zebrafish gfap promoterDepositorInsertgfap promoter
Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
Tags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W
Plasmid#67985PurposeCas9 activity reporter (control) with mCherry and BFPDepositorInsertU6gRNA cassette, PGKmCherryABFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorPublicationInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER13 miR-200c-141
Plasmid#81020PurposeLentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.DepositorPublicationInsertmiR-200c (MIR200C Human)
UseLentiviralAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Actin-cpstFRET-Actin
Plasmid#80643PurposecpstFRET actin sensor for mechanical force measurements in cytoskeletonDepositorInsertBeta actin, cpstFRET
ExpressionMammalianAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKA-141
Plasmid#79832PurposeExpresses RpBphP1-mCherryDepositorInsertBphP1-mCherry
ExpressionMammalianPromoterCMVd1Available sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLgw EcoDam-V5-RFC1
Plasmid#59209PurposeMammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseLentiviral; DamidTagsDam and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Rab9a
Plasmid#78592PurposeExpress mCherry-Rab9a in mammalian cellsDepositorAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pR26 GFP Dest
Plasmid#74283PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloningDepositorInsertRosa26 5-homology region
Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlineAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CASR-Tango
Plasmid#66235PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
