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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway destination vector

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-BFP (JDW 491)

Plasmid

#229834

Purpose

A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35R H43R SUMO1

Plasmid

#221063

Purpose

Expression of H35R H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R H43R double mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35R, H43R

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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small ubiquitin-like modifier 1 (SUMO1)

Plasmid

#221052

Purpose

Expression of small ubiquitin-like modifier 1 (SUMO1) protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTag4C_Nrg1-GV-2HA

Plasmid

#227104

Purpose

Nrg1 protein for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Nrg1-GV (Nrg1 Mouse)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0410-luc2

Plasmid

#227121

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0410

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0410 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0432-luc2

Plasmid

#227124

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0432

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0432 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0434-luc2

Plasmid

#227125

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0434

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0434 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0407-luc2

Plasmid

#227119

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0407

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0407 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0436-luc2

Plasmid

#227126

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0436

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0436 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0400-luc2

Plasmid

#227117

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0400

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0400 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0439-luc2

Plasmid

#227127

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0439

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0439 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0397-luc2

Plasmid

#227116

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0397

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0397 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0431-luc2

Plasmid

#227123

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0431

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0431 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBK_Gal4-DBD-Ar

Plasmid

#227098

Purpose

Ar receptor overexpression for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Ar (Ar Mouse)

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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