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We narrowed to 5,257 results for: phy

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Showing: 4341 - 4350 of 5257 results

  1. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185910-plasmid-map-sequence-id-363739

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  6. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  7. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

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  10. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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Showing: 4341 - 4350 of 5257 results