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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3

Plasmid

#110099

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPathBrick

Plasmid

#65006

Purpose

E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive native promoters

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 4

Plasmid

#99319

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO GFP-Zeo DEST (719-1)

Plasmid

#17431

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERN

Plasmid

#80475

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE5 CTFdeltaTA-FLAG

Plasmid

#217726

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE5 (ADGRE5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-536

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMRE145

Plasmid

#118497

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pYSD061

Plasmid

#212915

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB424

Plasmid

#74818

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE347

Plasmid

#153228

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

Plasmid

#217681

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB1 (ADGRB1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-926

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB540

Plasmid

#74874

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB504

Plasmid

#74846

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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p0521s

Plasmid

#62862

Purpose

Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

Plasmid

#217708

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL3 (ADGRL3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-841

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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