We narrowed to 10,277 results for: epo
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Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1063-luc2
Plasmid#227109PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1063DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
TagsLckExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_MX1
Plasmid#99323PurposeLuciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr21: 42791443 -42793515
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_IFIT2
Plasmid#99321PurposeLuciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr10: 91060605-91062447
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
Plasmid#85453PurposeLentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.DepositorInsertU6-sgRNA-Backbone-UP-TdTomato
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationdeletion of AA123-365 in tdTomato (please see dep…Available SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104095PurposeExpresses TREAT-siRNA reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianAvailable SinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7d-5p
Plasmid#103151PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7d-5p target (MIRLET7D Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
TagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HSPH2
Plasmid#19454DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
ExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…Available SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom108bp-pGL4.10Luc
Plasmid#60875PurposeLuc reporter driven by 108 bp of the CIP2A promoterDepositorInsertNM_020890108 bp promoter fragment of Cancerous Inhibitor of PP2A.2 (CIP2A Human)
UseLuciferase; PromoterlessAvailable SinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mIRFP670nuc-TEV-Xph20-ETWV
Plasmid#133044PurposeExpresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporterDepositorInsertXph20-ETWV
TagsNLS, TEV protease + TEV cleavage site, and miRFP6…ExpressionMammalianMutationS63KPromoterCAGAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR
Plasmid#53614PurposeLuciferase reporter assay for microRNA binding on the RECK 3'UTRDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-gp78-FLAG RING mutant C356G H361A
Plasmid#61751Purposeexpress C-terminally FLAG tagged human gp78DepositorInsertgp78 (AMFR Human)
TagsFLAGExpressionMammalianMutationC356G H361A; P179L, F262L, Y315C, and D346G (ple…PromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104094PurposeExpresses TREAT reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianPromoterTetCMVAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPV25-cR-2iG
Plasmid#204618PurposeFluorescent reporter for SPI2 induction in SalmonellaDepositorInsertsrpsM promoter
sseA promoter
TagsGFP and dsRedExpressionBacterialAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167943PurposeSFTPC reporter targeting repair templateDepositorInsertSFTPC (SFTPC Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterEF1aAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: pLX304-QuasAr2
Plasmid#62985PurposeLentivirus production of QuasAr2 fluorescent reporterDepositorInsertQuasAr2-mOrange
UseLentiviral; Gateway destination vectorTagsmOrange2ExpressionMammalianPromoterCMVAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only