We narrowed to 16,161 results for: nans

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pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

Plasmid

#160608

Purpose

TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

P35s:dCas9:SRDX:tNOS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2-pLenti6

Plasmid

#36950

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN2 (EGLN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Neurod1-3xT7

Plasmid

#200890

Purpose

Inducible lentiviral expression of Neurod1

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN3-pLenti6

Plasmid

#36951

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN3 (EGLN3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pcDNA3

Plasmid

#206072

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

Plasmid

#118278

Purpose

Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Insert

bPAC(WT)

Use

AAV

Tags

mCherry and myc

Promoter

CamKII

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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MYH11-gRNA1

Plasmid

#132587

Purpose

gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

MYH11-gRNA1

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV CytoBAS

Plasmid

#62860

Purpose

Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

CytoBAS

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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ins:GCaMP6s; cryaa:RFP

Plasmid

#110285

Purpose

A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

GCaMP6s

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LpIA(wild-type)

Plasmid

#61821

Purpose

Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

6XHis, EGFP, and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF52-2xCSTREP

Plasmid

#136212

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF52

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183536

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

None

Promoter

CaMKIIa

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Promoter

CamK II

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLpA-DN.JNK2

Plasmid

#51943

Purpose

Adenoviral vector for dominant negative JNK2 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK2 (Mapk9 Mouse)

Use

Adenoviral

Tags

HA

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pCMV NucleoBAS

Plasmid

#62861

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-EYFP

Plasmid

#35505

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130997

Purpose

Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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