We narrowed to 9,904 results for: UTY

1. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-NLS-R-GECO

   Plasmid

   #32462

   Purpose

   Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Use

   Tags

   NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX317-QKI

   Plasmid

   #115441

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   QKI (QKI Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PHB2 S39D-mCherry

   Plasmid

   #232787

   Purpose

   Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

   Depositor

   Giulia Bertolin

   Article

   Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

   Insert

   PHB2 (PHB2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Serine 39 to Aspartate

   Promoter

   CMV

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX317 TEAD2

   Plasmid

   #193686

   Purpose

   Constitutive lentiviral expression of TEAD2

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   TEAD2 (TEAD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mito-LAR-GECO1.2

   Plasmid

   #61245

   Purpose

   Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1.2
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1.2: N45I/A47R/E1…

   Promoter

   CMV

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

   Plasmid

   #123363

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   EGFP-NLS
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TACR1-DuET

   Plasmid

   #213385

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   TACR1 (TACR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX317-SNAI1

    Plasmid

    #115446

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    SNAI1 (SNAI1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid

    #131008

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV and pTRE3G-Bi

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MRGPRX1-DuET

    Plasmid

    #213344

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX1 (MRGPRX1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid

    #133733

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pGFAP and pTRE3G-Bi

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid

    #133732

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pCamk2a and pTRE3G-Bi

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX317-RBFOX1

    Plasmid

    #115442

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    RBFOX1 (RBFOX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194686

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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