We narrowed to 15,275 results for: grn

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-BaeI-Pgk-Cre

Plasmid

#173663

Purpose

Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

Depositor

Charles Swanton

Publication

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

BaeI site

Use

CRISPR, Cre/Lox, and Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#2

Plasmid

#174153

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TRCN0000338399

Plasmid

#78156

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Publication

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 2

Expression

Mammalian

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MSCV-PM mir shLEF1 b

Plasmid

#27018

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 1b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
PDG458 eSp(1.1) V3

Plasmid

#226964

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Publication

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRi-D2

Plasmid

#182927

Purpose

inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

Depositor

Himadri Pakrasi

Publication

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddcpf1

Use

CRISPR

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Publication

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSicoR-mCh-Chd1i4

Plasmid

#21908

Depositor

Miguel Ramalho-Santos

Publication

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i4

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pJZC548

Plasmid

#62316

Purpose

sgRNA with 1x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x PP7

Expression

Yeast

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V3

Plasmid

#226956

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDHODH-4

Plasmid

#186022

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Publication

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiCRISPR v2-sgLKB1 clone3

Plasmid

#162127

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RT3GEP

Plasmid

#111164

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Publication

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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