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We narrowed to 16,006 results for: grna

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Showing: 8721 - 8740 of 16006 results

  5. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  7. ATM N-terminal sgRNA

    Plasmid
    #207089
    Purpose
    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATCATTAAGTACTAGACTCA
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207089-plasmid-map-sequence-id-431774

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  9. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  10. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172835-plasmid-map-sequence-id-341082

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  11. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid
    #172825
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172825-plasmid-map-sequence-id-340913

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  12. pX330_rSyn1 sgRNA / hSpCas9

    Plasmid
    #172840
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172840-plasmid-map-sequence-id-340905

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  13. pX330_rActb sgRNA / hSpCas9

    Plasmid
    #172833
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172833-plasmid-map-sequence-id-340902

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  15. pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

    Plasmid
    #159792
    Purpose
    S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
    Expression
    Mammalian
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    159792-plasmid-map-sequence-id-314793

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  16. pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

    Plasmid
    #159791
    Purpose
    S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
    Expression
    Mammalian
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    159791-plasmid-map-sequence-id-314792

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Showing: 8721 - 8740 of 16006 results