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pDONR223-PHKB

Plasmid

#23859

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKB (PHKB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Publication

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Tags

His

Expression

Bacterial

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pEF 4r-GUS-3r

Plasmid

#20097

Depositor

Antonio Richart

Insert

GUS (gus )

Use

Synthetic Biology; Plant gateway vector

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFredA-PTTG1

Plasmid

#16584

Depositor

Bert Vogelstein

Publication

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

PTTG1 (PTTG1 Human)

Expression

Mammalian

Mutation

5' targeting element

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE D9A

Plasmid

#12686

Depositor

SeWon Suh

Publication

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 9 to Alanine

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
CMV-CyTERM-mTagBFP2-24xPP7

Plasmid

#254845

Purpose

an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of PP7 RNA tag

Depositor

Jiahui (Chris) Wu

Publication

Pham et al Nat Methods. 2026 Jan;23(1):165-174. doi

Insert

CyTERM-mTagBFP2-24xPP7

Expression

Bacterial

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-Puro

Plasmid

#253773

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-DEST-IRES-Hygro

Plasmid

#253775

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-Hygro

Plasmid

#253771

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MSTN

Plasmid

#241319

Purpose

Expresses bovine MSTN (wild-type) in mammalian cells

Depositor

Mathilde Dupont-Nivet

Publication

Gaiani et al Anim Genet. 2026 Feb;57(1):e70073. doi:

Insert

MSTN full gene (MSTN Bovine)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM045

Plasmid

#247633

Purpose

to generate mRNA for membrane mScarlet3 for zebrafish

Depositor

Gokul Upadhyayula

Publication

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mScarlet3

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Nucleocapsid

Plasmid

#242452

Purpose

Expresses the SARS-CoV-2 Nucleocapsid protein

Depositor

Benjamin Schuster

Publication

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

Nucleocapsid protein

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-meGFP-DLC1

Plasmid

#192048

Purpose

Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

Depositor

Ionel Popa

Publication

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

DLC1 (DLC1 Human)

Expression

Bacterial

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Calnexin-N-14

Plasmid

#231776

Purpose

Visualization of ER in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Calnexin-N-14 (CANX Human)

Tags

mGold2s

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i2-ipUSEPR-TR657

Plasmid

#228923

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i2 (Snrpa Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i4-ipUSEPR-TR657

Plasmid

#228924

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i4 (Snrpa Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLPR3

Plasmid

#209986

Purpose

Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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