159,237 results

1. PB-TRE-dCas9-KRAB-MeCP2

   Plasmid

   #122267

   Purpose

   SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   dCas9-KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQH

   Plasmid

   #48096

   Purpose

   Cumate-inducible expression of HA-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and HA

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-NEGR1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156728

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NEGR1 (NEGR1 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2_Firefly_degron-60A

   Plasmid

   #200396

   Purpose

   A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Firefly luciferase - degron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0723

   Plasmid

   #142226

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPROBE-gfp[AAV]

   Plasmid

   #40168

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotor probe
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. hSgk1_v2 Flag

   Plasmid

   #86040

   Purpose

   full length human Sgk1_i2 with Flag tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

   Insert

   Alternate transcript v2 of human Sgk1 (SGK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   v2

   Promoter

   CMV

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2095

   Plasmid

   #141918

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ST18 (ST18 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. BASIC_8_RBS34- mCherry -B0015

    Plasmid

    #68142

    Purpose

    storage vector for BASIC part: RBS-RFP(ORF)

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    RBS34-mcherry-B0015
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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