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SPHK2 gRNA (BRDN0001146882)

Plasmid

#77096

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK2 (SPHK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001148403)

Plasmid

#76815

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAT077

Plasmid

#180511

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
PlmCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 hFZD4

Plasmid

#115908

Purpose

Expresses human FZD4 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAdEasy-EGFP-CLASP2 340-1362

Plasmid

#24522

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGW-Dronpa-Cav1.2

Plasmid

#182333

Purpose

L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Cav1.2-Dronpa (CACNA1C Rabbit)

Tags

Full-length rabbit Cav1.2.

Expression

Mammalian

Mutation

T1066Y, Q1070M

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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VN-Sph1

Plasmid

#89755

Purpose

expresses human Synphilin fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

Insert

SNCAIP (SNCAIP Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#183809

Purpose

his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Article

Yu et al Science. 2022 Jul;377(6601):63-72. doi:

Insert

AAV transgene TetO promoter-DIO/flex-GCaMP6

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-MAP1LC3BK51A

Plasmid

#175754

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Mutation

K51A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GACh2.0

Plasmid

#121921

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor GACh2.0

Use

AAV

Promoter

hSyn

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-WT-T373A-S608A-S612A-puro

Plasmid

#212674

Purpose

Express tagged hRb mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

T373A/S608A/S612A

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SpRY_ATH51-IP

Plasmid

#235991

Purpose

Expresses SpRY_ATH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RPS15A-3xHA plasmid

Plasmid

#192245

Purpose

overexpression vector of RPS15A-3XHA

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

RPS15A CDS without stop coden (RPS15A Human)

Use

Lentiviral

Tags

3xHA

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMIA3-Pansio

Plasmid

#214445

Purpose

Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v2

Plasmid

#110070

Purpose

Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)

Tags

Halo-TEV-Flag

Expression

Mammalian

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-mNG

Plasmid

#183465

Purpose

To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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