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Plasmid#193310PurposeCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6_N7-sgRNA2
Plasmid#190664PurposeExpresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloningDepositorInsertN7 sgRNA2
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-NS
Plasmid#149545PurposeExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-U6insert
Plasmid#104536PurposeA piggybac vector with a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …TagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-AAVS1_hspCas9
Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKAR1/Pur
Plasmid#23105PurposeAllows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance geneDepositorTypeEmpty backboneUseRNAiTagsFlagExpressionMammalianMutationPromoterTRE for cDNA; U6 for shRNAAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA pAS single_hspCas9
Plasmid#193311PurposeCas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA pAS dual_hspCas9
Plasmid#193312PurposeCas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn3
Plasmid#59359PurposeLentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn1
Plasmid#59339PurposeLentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW:PtU6
Plasmid#104895PurposePhaeodactylum U6 promoterDepositorInsertPhaeodactylum U6 promoter
UseTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2102-reci
Plasmid#117171PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLSDepositorInsertreci_wtCas9 without NLS
UseLentiviralTagsExpressionMutationPromoterU6 PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2955
Plasmid#135589PurposeSNR6 has UUUAUUUC at position 100–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ8 has UUUAUUUC at position 100–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPS2956
Plasmid#135590PurposeSNR6 has GAUUUAUUUC at position 98–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ10 has GAUUUAUUUC at position 98–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only