We narrowed to 11,881 results for: pcDNA

1. pcDNA3.1-m-Flt4

   Plasmid

   #119232

   Purpose

   Plasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.

   Depositor

   Kari Alitalo

   Insert

   Vegfr3 (Flt4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-) FLIPQTV3.0 8m

   Plasmid

   #63732

   Purpose

   FLIPQTV3.0 glutamine sensor with R75MY86A mutations

   Depositor

   Sakiko Okumoto

   Article

   Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

   Insert

   FLIPQTV3.0 sensor, 8mM affinity
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-P4:gs:nls:VPR

   Plasmid

   #135983

   Purpose

   Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the VPR activation domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172228

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HA_human_BAP1

   Plasmid

   #158109

   Purpose

   Expresses tagged BAP1, neomycin selection

   Depositor

   Emanuela Felley-Bosco

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Letm1

   Plasmid

   #191962

   Purpose

   Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).

   Depositor

   Luca Scorrano

   Insert

   Letm1 (LETM1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-miR29b

   Plasmid

   #21121

   Depositor

   Joshua Mendell

   Article

   Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

   Insert

   miR-29b-1 (MIR29B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3.1neo-NLucYAP15

   Plasmid

   #107610

   Purpose

   measure LATS activity by Luciferase Assay

   Depositor

   Xiaolong Yang

   Article

   Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

   Insert

   NLucYAP15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-sACE2(WT)

   Plasmid

   #145147

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-R4-uAb

    Plasmid

    #101800

    Purpose

    Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Kv7.5/pcDNA3.1

    Plasmid

    #111498

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3_2FLAG-AKAP79ΔCN

    Plasmid

    #183451

    Purpose

    Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa337-343 deleted

    Promoter

    CMV

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 barr1 HA

    Plasmid

    #14693

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-HA-APC11

    Plasmid

    #19900

    Depositor

    Yue Xiong

    Article

    Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

    Insert

    APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NXPE1_pcDNA3.4-TOPO

    Plasmid

    #226841

    Purpose

    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.

    Depositor

    Bert Vogelstein

    Insert

    NXPE1 (NXPE1 Human)
    

    Use

    Tags

    x6 HIS and an IL2 secretion signal both N termina…

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-TRAIL-R1

    Plasmid

    #61382

    Purpose

    Expresses HA tagged TRAIL receptor 1

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 1
    

    Use

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R1 leader peptide (aa1-23) replaced with TN…

    Promoter

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-hBcan

    Plasmid

    #18966

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Jul 7. ():.

    Insert

    Brevican (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-hL1

    Plasmid

    #89411

    Purpose

    mammalian expression of hL1

    Depositor

    Fritz Rathjen

    Article

    De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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