2,226 results

1. LXSN-Cx43-S279/282A

   Plasmid

   #60713

   Purpose

   retroviral expression of rat Cx43 S279/282A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S279/282A

   Promoter

   LTR

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. LXSN-Cx43-S279/282D

   Plasmid

   #60715

   Purpose

   retroviral expression of rat Cx43 S279/282D mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S279/282D

   Promoter

   LTR

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FUP95PDZGW (C5)

   Plasmid

   #74021

   Purpose

   lentiviral expression of Psd95-EGFP fusion

   Depositor

   Oliver Schluter, Weifeng Xu, Robert Malenka

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PDZ

   Promoter

   hUbC

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

4. KIF5C(1-560)-mCitrine

   Plasmid

   #61676

   Purpose

   Single mCitrine-labeled, truncated kinesin-1

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KHC(1-560)-mCitrine (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEmerald-CathespinB-6

   Plasmid

   #54024

   Purpose

   Localization: Lysosomes, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CathespinB
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pcDNA5FRT/TO-p97-AE-mycStrep

   Plasmid

   #31841

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   A232E

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. mVenus-RnBarr1

   Plasmid

   #137796

   Purpose

   Visualization of beta-arrestin1

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin 1 (Arrb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KIF5C(1-560)-GFP11

   Plasmid

   #61670

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to split GFP linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-GFP11 (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOC4 Actb-mRuby3 KI

   Plasmid

   #183437

   Purpose

   FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and mRuby3 donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOC5 Tubb3-GFP KI

    Plasmid

    #183440

    Purpose

    FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Dronpa-PSD95-N-14

    Plasmid

    #57293

    Purpose

    Localization: Membrane Scaffold, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    PSD95
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pFastBac HT JS-Munc18b

    Plasmid

    #135554

    Purpose

    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18b (Stxbp2 Rat)
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDC183

    Plasmid

    #106415

    Purpose

    Co-expresses trans SNARE complex with pQZ210 in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    SNAP25 fragment 141-204 (Snap25 Synthetic, Rat)
    SNAP25 Fragment 7-83 (Snap25 Synthetic, Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fragment 141-204, Codon optimization and Fragment…

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFPC2-gephyrin S268A/270A

    Plasmid

    #199608

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268A/S270A

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FLAG-Fos AA

    Plasmid

    #8967

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362A, S374A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGP-CMV-GCaMP3-44

    Plasmid

    #91780

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-44
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI-neo-myc-LC3(deltaC22, G120A)

    Plasmid

    #45449

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Encodes amino acids 1-120, Glycine at position 12…

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLenti CMV GSE T329A Hygro (w189-4)

    Plasmid

    #22255

    Depositor

    Eric Campeau

    Insert

    Genetic suppressor element #22, T329A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 329 is mutated to Alanine (p53 nomencat…

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-dCpf1-BE0

    Plasmid

    #107678

    Purpose

    Expresses dLbCpf1-BE0 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

    Plasmid

    #32456

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S129A, S133A, S137A (S3V, G96R, and P108L also pr…

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

21. prNFL-Q333P-EGFP

    Plasmid

    #132602

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    998A>C, 999G>C; Gln333Pro

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEmerald-RatLC3B-C-7

    Plasmid

    #54147

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

    Insert

    RatLC3B
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. PA-mCherry-miniSOG-Cx43-7

    Plasmid

    #57787

    Purpose

    Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Cx43
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLL3.7 shRNA against CRTC3

    Plasmid

    #72712

    Purpose

    Lentiviral expression of shRNA against CRTC3 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC3
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA3.1 AT1AR-TSTS/4A

    Plasmid

    #45634

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-TSTS/A (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Thr332, Ser335, Thr336, and Ser338 all changed to…

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pVS45

    Plasmid

    #20059

    Depositor

    Stephen Sligar

    Article

    Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

    Insert

    rat hepatic cytochrome b5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

28. PS-CFP2-Cx26-7

    Plasmid

    #57222

    Purpose

    Localization: Gap Junctions, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    Cx26 (Gjb2 Rat)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. Rat NCX1.4 (pcDNA3.1+)

    Plasmid

    #75193

    Purpose

    Mammalian expression of SLC8A1

    Depositor

    Jonathan Lytton

    Article

    Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

    Insert

    SLC8A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Septin 11 isoform IV

    Plasmid

    #120320

    Purpose

    Septin 11 isoform IV mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge