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Showing: 861 - 880 of 4689 results

  1. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  4. pCFD5-NS

    Plasmid
    #149546
    Purpose
    Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149546-plasmid-map-sequence-id-306364

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  5. pCFD3-NS

    Plasmid
    #149545
    Purpose
    Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149545-plasmid-map-sequence-id-306357

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  8. pKAR1/Pur

    Plasmid
    #23105
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene
    Depositor
    Randy Poon
    Article
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    TRE for cDNA; U6 for shRNA
    Available since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pLenLox_U6 Nlgn3

    Plasmid
    #59359
    Purpose
    Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
    eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and mouse U6
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    59359-plasmid-map-sequence-id-92032

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  12. pLenLox_U6 Nlgn1

    Plasmid
    #59339
    Purpose
    Lentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    Neuroligin 1 shRNA (rat) (Nlgn1 Rat)
    eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and mouse U6
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    59339-plasmid-map-sequence-id-92033

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Showing: 861 - 880 of 4689 results