We narrowed to 17,086 results for: URE

1. pCW-GFP-CtIP-T847E

   Plasmid

   #71111

   Purpose

   mammalian expession of GFP-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. RP1B-PP1alpha

   Plasmid

   #51768

   Purpose

   Expresses PP1 alpha residues 7-330

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   pp1 alpha
   

   Use

   Tags

   His, TEV protease site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains PP1 alpha residues 7-330

   Promoter

   T7-lac

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. plc242-GFP-Nprl2

   Plasmid

   #87051

   Purpose

   mammalian expression of Nprl2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   Nprl2 (NPRL2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLQ-KO-tgt50

   Plasmid

   #126578

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b/BOA

   Plasmid

   #82041

   Purpose

   For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

   Depositor

   Angela Gronenborn

   Article

   Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

   Insert

   Burkholderia oklahomensis agglutinin
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-Flag-TRF2(delta)iDDR

   Plasmid

   #44577

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   TRF2ΔiDDR (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   iDDR domain (aa 407-431) has been deleted

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pOAD

   Plasmid

   #196825

   Purpose

   Yeast two-hybrid ; Gal4 activation domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 Activation domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFOSGE_COF4A02

   Plasmid

   #71021

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Ube3a (Ube3a Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCTCON2-GYB

   Plasmid

   #160927

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

    Plasmid

    #136544

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (29-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. hME-pOX

    Plasmid

    #178181

    Purpose

    Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

    Depositor

    Ming-Feng Tsai

    Article

    Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

    Insert

    human MCU-EMRE fusion protein
    

    Use

    Tags

    1D4

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

    Plasmid

    #136546

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (23-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. LacD.1_WT_pET21a

    Plasmid

    #29789

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124604

    Purpose

    Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Insert

    Soma-localized C1V1(t/t)
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFOSGE_COF3H08

    Plasmid

    #70996

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Nap1 (Nap1 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. PX543 SpCas9(del175-307)

    Plasmid

    #58888

    Purpose

    Truncated form of SpCas9 without REC2 domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 175-307

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-SZT2 1-1021

    Plasmid

    #87037

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 1-1021

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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