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pRS416-Aga1p-FAPB2.3.6

Plasmid

#158147

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Use

Tags

1D4

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

Plasmid

#136546

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Promoter

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV Sbf-Sal linker

Plasmid

#50434

Purpose

Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H08

Plasmid

#70996

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Nap1 (Nap1 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Promoter

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SZT2 1-1021

Plasmid

#87037

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1A01

Plasmid

#71088

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

pnr (pnr Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1A07

Plasmid

#71083

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

grn (grn Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2A03

Plasmid

#70878

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Elp3 (Elp3 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D06

Plasmid

#71048

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mor (mor Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3E07

Plasmid

#70987

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIS (TfIIS Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pTcI39-PRM-wasabi

Plasmid

#195555

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI39

Use

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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Interface-4 (b)

Plasmid

#204435

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

I735A

Promoter

CMV

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (b)

Plasmid

#204429

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

E626A T630A K668A E671A

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (c)

Plasmid

#204430

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q659A

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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