Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,133 results for: codon optimized

Clear keywords
Showing: 861 - 880 of 5133 results

  4. pAP-U2-1

    Plasmid
    #236472
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi
    Insert
    spH2Ap-3xmCherry spH2Bp-3xGFP
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236472-plasmid-map-sequence-id-468916

    Enlarge

  5. pAP-U2-3

    Plasmid
    #236474
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi
    Insert
    scACT1p-3xmCherry apACT1p-3xGFP
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236474-plasmid-map-sequence-id-468914

    Enlarge

  6. pAP-U2-4

    Plasmid
    #236475
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi
    Insert
    scACT1p-3xmCherry apTUB1p-3xGFP
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236475-plasmid-map-sequence-id-468488

    Enlarge

  7. pAP-U2-2

    Plasmid
    #236473
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi
    Insert
    spH2Bp-3xmCherry scACT1p-3xGFP
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236473-plasmid-map-sequence-id-468487

    Enlarge

  8. CSII-CAG-EGFP

    Plasmid
    #214112
    Purpose
    Mammalian continuous expression
    Depositor
    Sho Yoshimatsu
    Insert
    EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214112-plasmid-map-sequence-id-423582

    Enlarge

  9. pPB-CAG-Brainbow4.2-PGK-HygTK

    Plasmid
    #214126
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    Brainbow
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    214126-plasmid-map-sequence-id-428841

    Enlarge

  10. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Expression
    Yeast
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

    Enlarge

  11. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

    Enlarge

  12. pPB-hVASA-KO-WPRE

    Plasmid
    #214124
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    hKO
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214124-plasmid-map-sequence-id-424236

    Enlarge

  13. pLSU1/t35s[]:NLuc:NosT

    Plasmid
    #212180
    Purpose
    This binary vector expresses NanoLuciferase (Nluc) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    NanoLuc luciferase
    Expression
    Plant
    Mutation
    codon optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212180-plasmid-map-sequence-id-422749

    Enlarge

  14. pPB-cjOCT4-mNG-WPRE

    Plasmid
    #214123
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214123-plasmid-map-sequence-id-424234

    Enlarge

  15. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

    Enlarge

  16. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

    Enlarge

  17. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

    Enlarge

  18. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

    Enlarge

  19. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139988-plasmid-map-sequence-id-275338

    Enlarge

  20. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

    Enlarge

Showing: 861 - 880 of 5133 results