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We narrowed to 2,405 results for: dcas9

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Showing: 861 - 880 of 2405 results

  2. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  13. pCMV sMTase (eGFP)

    Plasmid
    #89930
    Purpose
    Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Publication
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    dCas9-MC-IRES-MN
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    deactivated Cas9
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89930-plasmid-map-sequence-id-178000

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  16. pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)

    Plasmid
    #250253
    Purpose
    Homology donor for knock-in of CRISPRi system into CLYBL locus with addgene plasmids 62196 and 62197. Lox-Stop-Lox requires cre delivery for activation.
    Depositor
    Jacob Corn
    Publication
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Insert
    dCas9-mTagBFP-KRAB
    Use
    CRISPR
    Tags
    HA-2xSV40 NLS-mTagBFP-KRAB
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Jan. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250253-plasmid-map-sequence-id-498726

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  17. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  20. pLV EF1a dCas9VP64 2A EGFP 2A BSD

    Plasmid
    #255458
    Purpose
    Expresses dCas9VP64, eGFP, and blasticidin
    Depositor
    Le Cong
    Insert
    dcas9-vp64
    Expression
    Mammalian
    Available since
    May 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    255458-plasmid-map-sequence-id-510788

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Showing: 861 - 880 of 2405 results