We narrowed to 1,266 results for: grna cloning vector

1. nzCas9n-GSG-linker-P2A-mNeongreen

   Plasmid

   #241189

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231363

   Purpose

   Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Banshee-ShCit-A

   Plasmid

   #85216

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Banshee-ShCit-B

   Plasmid

   #85217

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-B
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-2G-CNCB

   Plasmid

   #236764

   Purpose

   A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter, human U6 promoter, CAG promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-P2A-NTRv2-pA

   Plasmid

   #241195

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

   Depositor

   Junsu Kang

   Insert

   mCherry; NTR-v2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. linker-P2A-mem-mScarlet-ORF-3

    Plasmid

    #241185

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. linker-P2A-mem-mScarlet-ORF-2

    Plasmid

    #241184

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. linker-P2A-mem-mScarlet-ORF-1

    Plasmid

    #241183

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. linker-P2A-lifeact-mNeongreen-ORF-1

    Plasmid

    #241172

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. linker-P2A-lifeact-mNeongreen-ORF-2

    Plasmid

    #241173

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. linker-P2A-lifeact-mNeongreen-ORF-3

    Plasmid

    #241174

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

    Plasmid

    #192131

    Purpose

    Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

    Plasmid

    #225752

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

    Plasmid

    #225753

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SA-linker-P2A-mem-mScarlet-ORF-2

    Plasmid

    #241187

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SA-linker-P2A-mem-mScarlet-ORF-3

    Plasmid

    #241188

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge