We narrowed to 1,270 results for: grna cloning vector

1. pENTR-AmCyan

   Plasmid

   #138481

   Purpose

   For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   AmCyan
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG422_UV5

   Plasmid

   #165607

   Purpose

   Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   D1332K and R1333G mutations in SpCas9

   Promoter

   lacUV5 driving Cas9 KG and UV5 driving sgRNA

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SA-linker-P2A-mNeongreen-ORF1

   Plasmid

   #241208

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SA-linker-P2A-mNeongreen-ORF2

   Plasmid

   #241209

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SA-linker-P2A-mNeongreen-ORF3

   Plasmid

   #241210

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. nzCas9n-GSG-linker-P2A-mNeongreen

   Plasmid

   #241189

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-2G-CNCB

   Plasmid

   #236764

   Purpose

   A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter, human U6 promoter, CAG promoter

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Banshee-ShCit-B

    Plasmid

    #85217

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-B
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-P2A-NTRv2-pA

    Plasmid

    #241195

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

    Depositor

    Junsu Kang

    Insert

    mCherry; NTR-v2
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. linker-P2A-mem-mScarlet-ORF-3

    Plasmid

    #241185

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. linker-P2A-mem-mScarlet-ORF-2

    Plasmid

    #241184

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. linker-P2A-mem-mScarlet-ORF-1

    Plasmid

    #241183

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. linker-P2A-lifeact-mNeongreen-ORF-1

    Plasmid

    #241172

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. linker-P2A-lifeact-mNeongreen-ORF-2

    Plasmid

    #241173

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. linker-P2A-lifeact-mNeongreen-ORF-3

    Plasmid

    #241174

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sha2Cas9

    Plasmid

    #192140

    Purpose

    Expresses Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpeCas9

    Plasmid

    #192139

    Purpose

    Expresses SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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