We narrowed to 1,017 results for: hug

1. pLV[TetOn]-Puro-TRE3G>mAscl1

   Plasmid

   #198755

   Purpose

   Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RAB11A-GFP HDRT Source (pTR 143)

   Plasmid

   #112012

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-GFP HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. RAB11A-mCherry HDRT Source (pTR 144)

   Plasmid

   #112013

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-mCherry HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CD4-C-GFP HDRT Source (pTR 152)

   Plasmid

   #112018

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-GFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CLTA-GFP HDRT Source (pTR 153)

   Plasmid

   #112016

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CLTA-GFP HDRT (CLTA Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CLTA-mCherry HDRT Source (pTR 177)

   Plasmid

   #112017

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CLTA-mCherry HDRT (CLTA Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BATF-GFP HDRT Source (pTR 146)

   Plasmid

   #112015

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   BATF-GFP HDRT (BATF Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RAB11A-BFP HDRT Source (pTR 145)

   Plasmid

   #112014

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-BFP HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CD4-C-BFP HDRT Source (pTR 176)

   Plasmid

   #112020

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-BFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CD4-C-mCherry HDRT Source (pTR 175)

    Plasmid

    #112019

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-mCherry HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUB2.1

    Plasmid

    #40728

    Purpose

    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background

    Depositor

    Thomas Hughes

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cre Shine

    Plasmid

    #37404

    Purpose

    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.

    Depositor

    Thomas Hughes

    Insert

    Cre Shine
    

    Use

    Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-Tol2 transposase

    Plasmid

    #158774

    Purpose

    Expression of Tol2 transposase

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    Tol2 tranposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tol2 codon usage frequency well suitable for muri…

    Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcGFP-C2-hSphK2

    Plasmid

    #84370

    Purpose

    Expression of AcGFP-SphK2 fusion protein in mammalian cells

    Depositor

    Anthony Don, Hugh Rosen

    Article

    Don et al Biochem Biophys Res Commun. 2009 Feb 27;

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

    Plasmid

    #61257

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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