171,442 results
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Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2
Plasmid#171104PurposeExpresses sgRNA (ENO1 gRNA) in Candida auris.DepositorInsertsgRNA
ExpressionYeastPromoterADH1Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_IRF3_SMAD/FHA
Plasmid#178479PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p166
Plasmid#186180PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 166
UseSynthetic Biology; CloningAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorInsertCRISPRoff-TetOn
Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean
Plasmid#34910PurposepreDepositorHas ServiceAAV5Insertpresynaptic mGRASP
UseAAVPromoterCAGAvailable SinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
p256
Plasmid#186184PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 256
UseSynthetic Biology; CloningAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD
Plasmid#201170PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralMutationaa1-276 onlyPromoterTRE3GAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
Plasmid#64613Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF
Plasmid#101772PurposeExpresses EGFP tagged BAF in human cellsDepositorAvailable SinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E283 Hs.ROCK1
Plasmid#70567PurposeGateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)DepositorInsertROCK1 (ROCK1 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR4-YFP
Plasmid#13018DepositorAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralPromoterhEF1aAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
IDG_GPR89A_OE_1
Plasmid#161635PurposeOverexpresses GPR89ADepositorAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
Tagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTO135_pENO1-CaCas9-tCyc1
Plasmid#171103PurposeExpresses Cas9 in Candida aurisDepositorInsertCaCas9
ExpressionYeastPromoterENO1Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPR-A
Plasmid#89119Purposemammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933DepositorInserthuman progesterone receptor-A (PGR Human)
ExpressionMammalianMutationhuman progesterone receptor-A, equivalent to huma…PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNH605-HSE-YFP
Plasmid#172131PurposeFluorescent reporter for Hsf1 activityDepositorInsert4xHSE-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of the H…Available SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only