We narrowed to 18,162 results for: JUN
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Plasmid#103247PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-146a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-146a-3p target (MIR146A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
Plasmid#192445PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)
Plasmid#192444PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sh3bp2-SH2
Plasmid#46484DepositorInsertSh3bp2 (SH3BP2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-561PromoterTacAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDest27-mBIN1+17
Plasmid#61096PurposeExpress N-terminal GST tagged-mouse BIN1+17 in mammalian cellsDepositorInsertmouse BIN1 with exon 17, excluding exon 7, 11, 13-16
TagsGSTExpressionMammalianPromoterCMVAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-PHLDA3(wt)
Plasmid#72557PurposeHypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.DepositorAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187949PurposeSMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertSMASh-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1045C
Plasmid#125005PurposeExpresses gRNAs targeting hid, eve, and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fes-SH2
Plasmid#46426DepositorInsertFes (FES Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 396-497PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso2E335A
Plasmid#159588PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cellsDepositorAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del1 pET28a
Plasmid#122115PurposeExpression Brg1 amino acids 1-381 in bacteriaDepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Supt6h-SH2
Plasmid#46518DepositorInsertSupt6h (SUPT6H Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1317-1441PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-CK2alpha’ V67A/I175A (pJD24)
Plasmid#37363DepositorInsertCK2α' V67A/I175A (CSNK2A2 Human)
TagsHAExpressionMammalianMutationV67A, I175APromoterCMVAvailable SinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-296-3p
Plasmid#103387PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-296-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-296-3p target (MIR296 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
TagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-223-3p
Plasmid#103369PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-223-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-223-3p target (MIR223 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4t1 RARalpha (1-187)
Plasmid#35558DepositorAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck-SH2
Plasmid#46457DepositorInsertNck (NCK1 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 275-377PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tyk2-SH2
Plasmid#46527DepositorInsertTyk2 (TYK2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 452-567PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only