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pEM790

Plasmid

#242607

Purpose

T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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GAL-SUV39H2-L

Plasmid

#89136

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-8his

Plasmid

#111847

Purpose

Mammalian expression plasmid for soluble BG505 SOSIP.664

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 Env (BG505 SOSIP.664)

Tags

8his purification tag and CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; has SOSIP mutatio…

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pPlantPOP_STARBURST

Plasmid

#241284

Purpose

UBQ10 driven expression of STARBURST, a poly-cistronic fungal bioluminescense pathway, in plants

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

STARBURST

Expression

Plant

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHBS1449 SED1-Lenti

Plasmid

#180824

Purpose

SED1 biosensor for stable integration and expression in mammalian cells

Depositor

Rajat Rohatgi

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 osmosensor

Use

Lentiviral

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GW1-Peredox-mCitrine

Plasmid

#32386

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCLucIPZ

Plasmid

#53222

Purpose

Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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MI-Luciferase-IRES-mCherry

Plasmid

#75020

Purpose

Encodes the expression of Luciferase and mCherry

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

Inserts

firefly Luciferase
mCherry

Use

Luciferase and Retroviral

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Tet-ON

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-SV2A_STOP

Plasmid

#161423

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2A (SV2A Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pProEx-HTa-SOD1 WT

Plasmid

#191849

Purpose

express wild-type SOD1 in bacterial cells

Depositor

Nam-Chul Ha

Article

Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

Insert

SOD1 (SOD1 Human)

Expression

Bacterial

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Cys-60nmSAH-SPYcatcher

Plasmid

#214840

Purpose

This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-60nmSAH-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-P2A-BFP

Plasmid

#149447

Purpose

Expresses Cas9 and BFP in a lentiviral vector.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

P2A-BFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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