We narrowed to 10,500 results for: fus
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Plasmid#159282PurposeTo tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.DepositorInsertMecp2 (Mecp2 Mouse)
TagsNluciferase-P2A-TdTomatoExpressionMammalianMutationNluciferase-TdTomato fusionAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEC626
Plasmid#174367PurposeActivation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.DepositorInsertAlpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
UseSynthetic BiologyExpressionBacterialPromoterJ23106Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
TagsHis-SUMOExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF251
Plasmid#101451PurposeDonor Vector containing ZNF251 transcription factor, part of the Human TFome CollectionDepositorInsertZNF251 (ZNF251 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HES3
Plasmid#101411PurposeDonor Vector containing HES3 transcription factor, part of the Human TFome CollectionDepositorInsertHES3 (HES3 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HES5
Plasmid#101409PurposeDonor Vector containing HES5 transcription factor, part of the Human TFome CollectionDepositorInsertHES5 (HES5 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC1-mCherry-H2B-Venus
Plasmid#129559Purposenuclear markerDepositorInsertmcherry and Histone 2B fused to venus
ExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP1
Plasmid#160084PurposeBacterial expression of Tn5-APEX2 fusion protein, no linkerDepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP2
Plasmid#160085PurposeBacterial expression of Tn5-APEX2 fusion protein with linker PAPAPDepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:FKBP
Plasmid#135984PurposeExpresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the FKBP domain
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TKN-bp1
Plasmid#133428PurposeHuman BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.DepositorAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)PromoterT7Available SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114105PurposeCre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVExpressionMammalianPromoterEF1alphaAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
TagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoAC
Plasmid#100032PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.DepositorInsertRhoAC
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationE497K/C566DPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC
Plasmid#100031PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.DepositorInsertRhoGC
Tags1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)ExpressionMammalianPromoterAdenovirus major late promoterAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBMN GBRP
Plasmid#89293PurposeFor expression of the mammalian Atg8 protein GABARAP without a tagDepositorAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Or45b-Gal4
Plasmid#63036PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or45b, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or45b promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or74a-Gal4
Plasmid#63048PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or74a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or74a promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only