We narrowed to 11,081 results for: aga

Showing: 8841 - 8860 of 11081 results

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pDECKO_GFP

Plasmid

#72619

Purpose

Expresses two gRNAs targeting GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward GFP

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-BirA-HA

Plasmid

#232587

Purpose

Express BirA tagged to the C-terminus of CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade

Plasmid

#140622

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTC391

Plasmid

#91220

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmIL-6 mut NF-kB

Plasmid

#61293

Purpose

drives luciferase from mouse IL-6 promoter with mutation in NF-kB site

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Mutated NF-kB binding site from GTGGGATTTTCCCA to…

Promoter

IL-6 promoter with mutant NF-kB binding site

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pU6_(GLuc)_INT[3xMS2_SL]

Plasmid

#68426

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three MS2 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCR1281

Plasmid

#111124

Purpose

Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC3 gRNA (XRCC3 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-Bos MAL Flag

Plasmid

#41554

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

MAL (TIRAP Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pDECKO_Malat1_A

Plasmid

#72620

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCR1319

Plasmid

#111116

Purpose

Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T gRNA (UBE2T Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-1-155-Ggamma2

Plasmid

#234752

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNG2 (GNG2 Human)

Tags

VC155

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-155-239-Gbeta1

Plasmid

#234751

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNB1 (GNB1 Human)

Tags

VN155

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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