We narrowed to 8,607 results for: Ott;

1. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

   Plasmid

   #47457

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

   Plasmid

   #61406

   Purpose

   Expresses human OTUD1 (OTU+UIM domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. JI401: Ad5 E1A/Hexon qRT-PCR standard

   Plasmid

   #131753

   Purpose

   Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Inserts

   Ad5 E1A gene fragment
   Ad5 Hexon gene fragment
   

   Use

   Standard for qrt-pcr

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78304

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLINTR mCHERRY v1 backbone

   Plasmid

   #179778

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAIP-hXRCC1pd-eGFP

   Plasmid

   #206033

   Purpose

   Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

   Promoter

   CAG

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)mGAT1-0-GFP

   Plasmid

   #41662

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal hydrophobic isoleucine residue added a…

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. KK601: pMVP (L3-L2) ecDHFR DD + polyA

   Plasmid

   #121790

   Purpose

   pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   ecDHFR Degron Domain + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR-SF61

    Plasmid

    #163916

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277

    Insert

    SF5Phe tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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