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pICH47802-p35S::tdTomato-ER::tNOS

Plasmid

#160220

Purpose

tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

p2x35S::TdTomato-ER::tNOS

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-Vinculin-23

Plasmid

#55516

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vinculin (VCL Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
p35S-GW-3Avi

Plasmid

#127918

Purpose

Gateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Type

Empty backbone

Tags

3xAviTag

Expression

Plant

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA_sh_resistant

Plasmid

#105006

Purpose

Mammalian Expression of PDZD8, sh resistant

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Mutation

The sequence recognized by shPDZD8 (TRCN000043105…

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP1_G1

Plasmid

#127114

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M47)

Plasmid

#23286

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax(M47)

Expression

Mammalian

Mutation

L319R L320S

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-NKX2.2

Plasmid

#134670

Purpose

Retroviral expression of mouse Nkx2.2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad2

Plasmid

#15722

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 shRNA (SMAD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-2-84-FLAG

Plasmid

#35980

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 84 amino acids

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK TT RBFOX2

Plasmid

#59770

Purpose

doxycycline-inducible expression of untagged human RBFOX2 in mammalian cells

Depositor

Phil Sharp

Article

Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

Insert

RBFOX2 (RBFOX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBirA-P19

Plasmid

#127919

Purpose

Transient expression of biotin ligase BirA and p19 in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

BirA

Expression

Plant

Promoter

35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2(E488Q)

Plasmid

#124621

Purpose

Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2(E488Q)

Use

AAV

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP6450

Plasmid

#128731

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

Insert

HopAD1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDB22

Plasmid

#61342

Purpose

Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-cMyc1A NLS and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII

Plasmid

#45064

Purpose

Expression of CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Promoter

RSV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bik

Plasmid

#10952

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

human Bik gene (BIK Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag-SIRT1 H355A

Plasmid

#8439

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-CSF2-218-PDGFR

Plasmid

#139058

Purpose

A lentivirus vector to integrate a surface membrane anchored human CSF2 gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

colony stimulating factor 2 (CSF2) (CSF2 Human)

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SRY_P2A_Hygro_Barcode

Plasmid

#120485

Purpose

Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SRY (SRY Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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