We narrowed to 10,006 results for: Gnas

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GINS1 D11.1 gRNA

Plasmid

#90694

Purpose

3rd generation lentiviral gRNA plasmid targeting human GINS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GINS1 (Guide Designation D11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mCherry Gamma 3

Plasmid

#65234

Purpose

To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

Depositor

Narasimhan Gautam

Article

Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

Inserts

mcherry
Gng3 (Gng3 Mouse)

Tags

6-His tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-AVITEV-GFP

Plasmid

#74052

Purpose

retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

AVITEV-GFP

Use

Retroviral

Tags

AVI-TEV-tag N-terminal to the GFP

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA:Asip

Plasmid

#60968

Purpose

Expression vector of rat Asip guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

Agouti signaling protein gRNA (Asip Rat)

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(C631S, C643S and C699T)

Plasmid

#52128

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

C631S, C643S and C699T

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 T7 Jip1b(471-707)

Plasmid

#52428

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-470

Available Since

April 29, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(282-707)

Plasmid

#52126

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pEBG MKK3b (Glu)

Plasmid

#50451

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Gst

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

SV40

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pET-MRPL15

Plasmid

#31345

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L15 mature form (MRPL15 Human)

Tags

His

Expression

Bacterial

Mutation

Has the amino acids in the predicted mature form …

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4-rB90

Plasmid

#18959

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican C-terminus (Bcan Rat)

Expression

Mammalian

Mutation

Rat brevican C-terminus: Signal peptide aa 1-22 …

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-PKAsub(T/A)-YPet-NES

Plasmid

#138205

Purpose

Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub(T/A)-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub(T/A)-RLuc8N-NES

Plasmid

#138212

Purpose

Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub(T/A)-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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enDR3-TEV-mEGFP-NLS (pRS1451)

Plasmid

#240546

Purpose

Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

Depositor

Roman Szczęsny

Article

Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

enDR3

Expression

Mammalian

Mutation

L52M

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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L4300 hIL10 in pcDNA backbone

Plasmid

#244185

Purpose

Constitutive expression of human IL-10 (with its signal sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

IL-10 (IL10 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TA1-DuET

Plasmid

#213382

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

TA1 (TAAR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-Flp

Plasmid

#149296

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-Flp

Plasmid

#149294

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHW581

Plasmid

#198818

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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