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pLUM

Plasmid

#64169

Purpose

harbours marker genes LEU2, URA3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CRISPRmTmG2

Plasmid

#69992

Purpose

The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

Depositor

Connie Cepko

Publication

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA that targets near LoxP sites

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAM3110

Plasmid

#40247

Depositor

Susan Golden

Publication

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

attP Site

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-EGFPFL

Plasmid

#46310

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFPFL DNA-1
linker
EGFPFL DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-EGFP100

Plasmid

#46309

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFP100 DNA-1
linker
EGFP100 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 W436R

Plasmid

#11018

Depositor

Matthew Meyerson

Publication

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 W436R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

W436R

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Tom70-NovoTag657(cv)

Plasmid

#255880

Purpose

Expression plasmid for NovoTag657(cv), a NovoTag657 variant for covalent binding, fused to the mitochondrial localization signal Tom70

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoTag657(cv)

Tags

Tom70

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3

Plasmid

#244224

Purpose

Vector for cloning LbCas12a crRNA array libraries. Contains 3' TruSeq sequence for easy NGS library construction

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK(D110A)

Plasmid

#241242

Purpose

Negative control viral expression vecotor for plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Lck-mCherry-ibARK(D110A)

Use

AAV

Mutation

D110A

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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His-huH2Ax(T137E_A139D_Q141E)-huH2B

Plasmid

#197273

Purpose

Human (hu) histones H2Ax and H2B inserted into a plasmid. Contains the phosphomimetic substitutions T137E, A139D, and Q141E in H2Ax.

Depositor

Mark Glover

Publication

Panigrahi et al Nucleic Acids Res. 2025 Oct 14;53(19):gk

Inserts

huH2Ax
huH2B

Tags

Histidine tag and No tag

Expression

Bacterial

Mutation

T137E, A139D, and Q141E in H2Ax

Available since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHM093

Plasmid

#225525

Purpose

Encodes rtTA under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Publication

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

rtTA
BFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-PEX3-EGFP

Plasmid

#228142

Purpose

Expresses PEX3-GFP in mammalian cells

Depositor

Noboru Mizushima

Publication

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

PEX3 (PEX3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHM039

Plasmid

#225523

Purpose

Encodes Wnt-responsive rtTA and constitutive BFP

Depositor

Jared Toettcher

Publication

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

rtTA
BFP

Expression

Mammalian

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-mTagBFP2-Cre (JDW 918)

Plasmid

#224517

Purpose

A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-mTagBFP2-Cre

Use

Cre/Lox

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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HTR2A-DuET

Plasmid

#213321

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HTR2A (HTR2A Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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HCTR2-DuET

Plasmid

#213311

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HCTR2 (HCRTR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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