We narrowed to 11,803 results for: nsf

1. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

   Plasmid

   #114375

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv2.1C (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Kv2.1C

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

   Plasmid

   #114372

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Nlgn1C (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Nlgn1C

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMflCT-o4

   Plasmid

   #101313

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   cat resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMflT-o3

   Plasmid

   #101311

   Purpose

   Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2023

   Plasmid

   #49130

   Purpose

   AAV plasmid with hs1218 promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-hs1218-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hs1218

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEMS2024

   Plasmid

   #49131

   Purpose

   AAV plasmid with hs671 promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-hs671-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hs671

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEMS2050

   Plasmid

   #49135

   Purpose

   AAV plasmid with CAGGS promoter driving expression of EmGFP.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-emGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUASp-Flag-Myc-Qin-Tudor-attB

   Plasmid

   #44210

   Depositor

   Phillip Zamore

   Article

   Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

   Insert

   Qin-Tudor-domain (qin Fly)
   

   Use

   Fly transformation

   Tags

   Flag and Myc

   Mutation

   lacks E3 domain on N-term (del aa1-274)

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-32a(+)-TGFB1m

    Plasmid

    #120286

    Purpose

    Produce human recombinant mTGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β1
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promotor

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-32a(+)-TGFB3m

    Plasmid

    #120289

    Purpose

    Produce human recombinant mTGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β3
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Kevin Brindle, Duncan Jodrell

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1 and PGK

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

    Plasmid

    #190163

    Purpose

    Microglia-targeted transgene expression.

    Depositor

    Hirokazu Hirai

    Article

    Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

    Inserts

    mIba1 promoter, 1.7-kb, microglia-specific promoter
    miR-9-Tx4, 4 repeated miRNA target sites for miR-9
    miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
    

    Use

    AAV

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynapsin1.OxLight1

    Plasmid

    #169792

    Purpose

    Fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight1
    

    Use

    AAV

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV INS2x mRFP-3MREs (AAV-beta)

    Plasmid

    #206928

    Purpose

    Beta cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Article

    Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    INSx2

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Promoter

    EF1a and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPCX-Cx43-IRES-GFP

    Plasmid

    #65433

    Purpose

    Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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