We narrowed to 9,399 results for: pho;
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Plasmid#168266PurposeA single-component optogenetic tool for photoswitchable pyroptosisDepositorInsertmGSDMD(1-276) (Gsdmd Mouse)
TagscpLOV2, mCherryExpressionMammalianMutationR138A, K146A, R152A, R154APromoterCMVAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-INPP4B
Plasmid#116864PurposeRecruitable PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI W391F V398L
Plasmid#60787PurposeExpresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI W391F V398L
TagsNuclear Export SignalExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
Tags6xHis and MBPExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-DIO-mCReP
Plasmid#167503PurposeConditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)DepositorAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
TagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-INPP4B-CAAX
Plasmid#116857Purposeplasma membrane-targetted PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
S1PR1-DuET
Plasmid#213374PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-mTurq2-Apollo-NADP+
Plasmid#193303PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the mitochondriaDepositorInsertG6PD (G6PD Human)
TagsmTurqoise2 and mitochondrial targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEosEM-E_Lamin A
Plasmid#226338PurposePhotoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expressionDepositorAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-Mito-7
Plasmid#57189PurposeLocalization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517DepositorAvailable SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165491PurposeNeuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .DepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseAAVTagsVenusExpressionMammalianPromoterhSyn1Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-G446E
Plasmid#25643DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralExpressionMammalianMutationtumor-derived mutation created by site-directed m…Available SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Sema7a-Fc-His
Plasmid#72175PurposeExpresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mRuby3-6xFLAG
Plasmid#161613PurposeCell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.DepositorInsertmRuby3-6xFLAG
UseAAVExpressionMammalianMutationN/APromoterHuman synapsin 1 promoterAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only