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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRSII42N-TDH3pr

Plasmid

#194531

Purpose

High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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PKC beta I DN

Plasmid

#16381

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFCyR2

Plasmid

#167513

Purpose

Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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T10-T7-hMOV10

Plasmid

#185052

Purpose

expression of WT human MOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Tags

T7

Expression

Mammalian

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-RFP

Plasmid

#26753

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Centrin 4 (cetn4 Frog)

Use

Reporter for basal body

Tags

monomeric RFP

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pBLxG2

Plasmid

#149472

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR6

Plasmid

#149493

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRXTN

Plasmid

#47916

Purpose

pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.

Depositor

James Iglehart

Article

Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

Tet

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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negAKARet-cyto

Plasmid

#114733

Purpose

Negative control of AKARet-cyto

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

Negative Control of AKARet-cyto

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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OmpX

Plasmid

#213136

Purpose

Expresses outer membrane protein X

Depositor

Sebastian Hiller

Article

Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

Insert

OmpX (24-171) (ompX E.coli (K12))

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mCherry

Plasmid

#87664

Purpose

retroviral vector encoding cre dependent expression of membrane mCherry

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

Mem-mCherry

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAM2314-RbcS-mNG

Plasmid

#165582

Purpose

Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen

Expression

Bacterial

Promoter

Prbcs

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFCiKm7

Plasmid

#167521

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-KmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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PKC gamma DN

Plasmid

#21239

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

K380R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PKC gamma WT

Plasmid

#21236

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pRS-shTGFbeta1

Plasmid

#14973

Depositor

Joan Massague

Article

Thomas et al Cancer Cell. 2005 Nov . 8(5):369-80.

Insert

TGFbeta1 shRNA (Tgfb1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only