19,824 results

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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-S(4+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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AsCas12a-6xNLS-E174R/S542R (pRG232)

Plasmid

#149723

Purpose

Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTRETightBI-RY-0

Plasmid

#31463

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Tags

NLS

Expression

Mammalian

Available Since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA Set9 wt

Plasmid

#24084

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Tags

MPB

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Lhx2

Plasmid

#61537

Purpose

Expresses Lhx2 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Lhx2 (Lhx2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO GFP-Zeo DEST (719-1)

Plasmid

#17431

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8m-WPRE

Plasmid

#176751

Purpose

AAV-mediated expression of GCaMP8m under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

CamKIIalpha

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1309

Plasmid

#16509

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 1309 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pAKlux2

Plasmid

#14080

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Promoter

Lac, T3

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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