We narrowed to 9,707 results for: crispr plasmids

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pAC573

Plasmid

#127974

Purpose

AMA1 plasmid with argB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

argB

Use

Fungal expression

Expression

Bacterial

Promoter

native promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pWT019b

Plasmid

#107894

Purpose

W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-dsRed

Plasmid

#217006

Purpose

lentiviral plasmid for gRNA cloning with selectable dsRed expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-CR-PARK7WT-VA

Plasmid

#115187

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

c.150G>A (silent when translated to protein, c…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-CR-PARK7V51G-VA

Plasmid

#115188

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

c.150G>A (silent when translated to protein, c…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-CR-PARK7C53A-VA

Plasmid

#115189

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

c.150G>A (silent when translated to protein, c…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-CR-PARK7H126A-VA

Plasmid

#115190

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

c.150G>A (silent when translated to protein, c…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-CR-PARK7E163K-VA

Plasmid

#115191

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

c.150G>A (silent when translated to protein, c…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-641 JE-TRAC-BCMA(BB)28z-P2A

Plasmid

#186127

Purpose

BCMA-CAR DNA production

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BCMA-CAR (TNFRSF17 Human)

Use

CRISPR

Mutation

WT

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Hygro)

Plasmid

#158622

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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AP1muA-mStayGold-polyA-Hygro HR

Plasmid

#229679

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

monomeric StayGold

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-601_NY-ESO-1_TCR_GFP

Plasmid

#207482

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

GFP, NY-ESO-1_TCR

Use

CRISPR

Mutation

Potential silent point mutation in GFP sequence

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY093-EFS-BCMA-Blast-WPRE

Plasmid

#192194

Purpose

Lenti-BCMA-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJH127

Plasmid

#162483

Purpose

SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJH130

Plasmid

#162484

Purpose

SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBFC0993

Plasmid

#231993

Purpose

CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

Depositor

Brady Cress

Article

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Inserts

dRfxCas13d
crRNA with negative control (RFP-targeting) spacer

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and pTet

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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