We narrowed to 36,889 results for: kan

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Lyso-RA

Plasmid

#209875

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-mCherry-FRB*

Plasmid

#207145

Purpose

Lysosome-targeted rapalog dimerizer

Depositor

Antony Galione

Publication

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

LAMP1 (LAMP1 Human)

Tags

FRB* (T2098L mutant of FKBP-rapamycin binding dom…

Expression

Mammalian

Mutation

Silent mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Publication

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo (HPRT1 Human)

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0047

Plasmid

#205139

Purpose

Expression of 6xHis-EGFP-2xPX in bacteria

Depositor

Jason King

Publication

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

6xHis

Expression

Bacterial

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C-GFP

Plasmid

#195154

Purpose

image COR1C

Depositor

Gia Voeltz

Publication

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Expression

Mammalian

Mutation

mutations to make si RNA resistant

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pJAM1.21_PB-UGCG-PGK-Blast

Plasmid

#205566

Purpose

Express human UGCG

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

UGCG (UGCG Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1186 Lyn11-ECFP-FIREmate

Plasmid

#205168

Purpose

Expresses Lyn11-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Publication

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

Lyn11-ECFP-FIREmate

Expression

Mammalian

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1169 FIREmate-ECFP-Cb5

Plasmid

#205167

Purpose

Expresses FIREmate-ECFP-Cb5 in mammalian cells

Depositor

Arnaud Gautier

Publication

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Cb5

Expression

Mammalian

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Publication

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pH-ABE8e

Plasmid

#200774

Purpose

For plant adenine base editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

Insert

nCas9(D10A)-TadA8e

Use

CRISPR

Expression

Plant

Mutation

D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

Promoter

maize Ubiquitin-1, OsU3

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2B

Plasmid

#202728

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (PIP4K2B Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx1

Plasmid

#202751

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170):mNG2 (PLCD1 Human)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4C

Plasmid

#202680

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Publication

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP670-PIP4K2C

Plasmid

#202750

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP670:PIP4K2C (PIP4K2C Human)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Publication

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pARSCL504

Plasmid

#198636

Purpose

Expresses a transgene encoding RUBY

Depositor

Shaun Curtin

Insert

35S:Ω:Ruby

Expression

Plant

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Publication

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTHmut

Plasmid

#200159

Purpose

For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Depositor

Kate Meyer

Publication

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHmut

Tags

6xHis, HA, and MBP

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-SUMO-BG2.4

Plasmid

#199438

Purpose

expresses BAmGreen2.4 in bacterial cells with a SUMO tag

Depositor

Andrew Woolley

Publication

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen2.4

Tags

his tag and sumo

Expression

Bacterial

Promoter

T7

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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