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pCMV-myc-Atg5

Plasmid

#24922

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

Atg5 (ATG5 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pTXB1-TnY

Plasmid

#163975

Purpose

IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

Depositor

Peter Smibert

Article

Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

TnY

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCCAT1-Red

Plasmid

#226521

Purpose

CCAT1 transgene with fluorescent protein mKate2

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

EF1a_mKate2_CMV_CCAT1 (CCAT1 Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAL005

Plasmid

#233865

Purpose

MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2

Plasmid

#17793

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pKD077

Plasmid

#136473

Purpose

SSB-mTur2 IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-mTur2 (ssb E. coli)

Tags

mTurquoise2

Expression

Bacterial

Mutation

mTurquoise2 inserted between Phe148 and Ser149

Promoter

T7 promoter

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR-SF61

Plasmid

#163916

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277

Insert

SF5Phe tRNA synthetase

Expression

Bacterial

Promoter

araBAD

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47869

Purpose

All-in-one plasmid targeting tdTomato

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
tdTomato crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-ErbB4CTF

Plasmid

#17803

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

HA

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-BFP2-(mCherry)'

Plasmid

#115235

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Expression

Yeast

Promoter

VTC4 (native promoter)

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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