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pcDNA3-Xt-VSP

Plasmid

#51887

Purpose

expression of Xt-VSP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

see comments*

Promoter

CMV

Available Since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL.gp91.Syn.Intron.eGFP

Plasmid

#30467

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

gp91/Syn.Intron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-MmUbc7/2783

Plasmid

#11490

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pDH003

Plasmid

#157640

Purpose

sMHT-113 fused to SYNZIP17/18

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP2

Plasmid

#51885

Purpose

expression of Xl-VSP2 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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p5xUAS

Plasmid

#165891

Purpose

Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xUAS

Expression

Insect

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p5xQUAS

Plasmid

#165818

Purpose

Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xQUAS

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ414-F13

Plasmid

#23056

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pEnt L1L3-MCS

Plasmid

#26798

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Available Since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Tags

His and mOrange

Expression

Bacterial

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGe1-V5His6_A

Plasmid

#145892

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pVP16 AD

Plasmid

#165822

Purpose

VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

VP16 AD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_3

Plasmid

#26705

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Expression

Bacterial

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#165856

Purpose

Universal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAdh 3'UTR Short

Plasmid

#165800

Purpose

Drosophila HSP70B 3'UTR sequence

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70 3'UTR

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmHsp70b

Plasmid

#165785

Purpose

Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3-Hsp70b

Plasmid

#165773

Purpose

HSP70B Promoter fused to synthetic 3xP3 eye driver

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3x3-Hsp70b

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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5xGMR

Plasmid

#165775

Purpose

5xGMR syntethic eye driver enhancer, requires minimal promoter element

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xGMR

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3

Plasmid

#21712

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Enhancer of decapping 3 (Edc3 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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YFP-Gamma 9-3

Plasmid

#36074

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein G9(gamma 3 tail)

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pHSP70B-CP6-hph

Plasmid

#165879

Purpose

Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A-invitro(KaganE12)

Plasmid

#52016

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Expression

Mammalian

Mutation

changed Met 1 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-G67M-invitro(KaganD100)

Plasmid

#52014

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

Changed Gly 67 to Met

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-L62M-invitro(KaganD98)

Plasmid

#52013

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

Changed Leu 62 to Met

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-E80M-invitro(KaganD99)

Plasmid

#52015

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

Changed Glu 80 to Met

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only