We narrowed to 9,389 results for: Coli

1. pXD70LacZ9.1-HcdR-Phcdh-LacZ

   Plasmid

   #191629

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNB201

   Plasmid

   #169112

   Purpose

   TESEC expression vector for Alr from M. tuberculosis

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   alr (alr Mycobacterium tuberculosis H37Rv)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pBAD

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNB202

   Plasmid

   #169113

   Purpose

   TESEC expression vector for mEGFP

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   mEGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pBAD

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCASCADE-xylA

   Plasmid

   #158611

   Purpose

   Low phosphate inducible gRNA silencing the xylA promoter

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   xylA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDF-NtXNtS1HR1AtR2NtB2

   Plasmid

   #160890

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-S1H
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-SH1: T7, chaperones: T13

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDF-NtXNtT4aR1AtR2NtB2

   Plasmid

   #160887

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-T4a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-T4a: T7, chaperones: T10

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EcNusGKOW

   Plasmid

   #113124

   Purpose

   Expresses C-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-124

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EcRfaHKOW

   Plasmid

   #113125

   Purpose

   Expresses C-terminus of E. coli RfaH

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15-His-∆NGlpG(Y205F)

   Plasmid

   #155132

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Y205F, insert codes for GlpG residues 87-276

   Promoter

   T7

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACYCDuet_M42FDHFR_WTTS

    Plasmid

    #91237

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M42F

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACYCDuet_M42FG121VDHFR_R166QTS

    Plasmid

    #91238

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M42FG121 and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACYCDuet_M42FG121VDHFR_WTTS

    Plasmid

    #91239

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M42FG121V

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACYCDuet_W22HDHFR_R166QTS

    Plasmid

    #91240

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R166Q and W22H

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACYCDuet_D27NDHFR_R166QTS

    Plasmid

    #91243

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D27N and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACYCDuet_F31VDHFR_R166QTS

    Plasmid

    #91245

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31V and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACYCDuet_F31YG121VDHFR_R166QTS

    Plasmid

    #91228

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31YG121 and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACYCDuet_F31YL54IDHFR_R166QTS

    Plasmid

    #91230

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31YL54I and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACYCDuet_F31YL54IDHFR_WTTS

    Plasmid

    #91231

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31YL54I

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACYCDuet_G121VDHFR_R166QTS

    Plasmid

    #91232

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G121V and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACYCDuet_L54IDHFR_R166QTS

    Plasmid

    #91234

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L54I and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACYCDuet_L54IDHFR_WTTS

    Plasmid

    #91235

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L54I

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACYCDuet_M42FDHFR_R166QTS

    Plasmid

    #91236

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M42F and R166Q

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVR2-m2

    Plasmid

    #84721

    Purpose

    pVR2 but with a G to U mutation in the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G to U mutation at position 16 of annotated "…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVR2-m3

    Plasmid

    #84722

    Purpose

    pVR2 but with a C to G mutation in the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C to G mutation at position 22 of annotated "…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVR2-m1

    Plasmid

    #84720

    Purpose

    pVR2 but with a C to U mutation in the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C to U mutation at position 13 of annotated "…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET22b-ecDHFR-dCys

    Plasmid

    #110541

    Purpose

    Bacterial protein of E.coli DHFR C85A/C152S mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C85 mutated to A85, C152 mutated to S152

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSMART-ala13

    Plasmid

    #87174

    Purpose

    NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Constitutive proD

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSMART-ala11

    Plasmid

    #87172

    Purpose

    NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Constitutive proA

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSMART-ala12

    Plasmid

    #87173

    Purpose

    NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Constitutive proC

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHL 1410

    Plasmid

    #53011

    Purpose

    pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    DsrA sRNA
    ompC
    rpoS
    

    Use

    Synthetic Biology

    Tags

    T7noRBS (900bp) and gfp

    Expression

    Bacterial

    Mutation

    contains −150 to +30 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pHL 762

    Plasmid

    #52990

    Purpose

    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    MicC sRNA
    sodB
    ompC
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    −56 to +141 bp and −81 to +36 bp

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pHL 1158

    Plasmid

    #53007

    Purpose

    pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    DsrA sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains -56 to +141 relative to start codon and …

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pHL1529

    Plasmid

    #53027

    Purpose

    PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    RBS(st2), RBS(st3), and glgC leader contains -61 …

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind and pLlac0-1

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pPK1E-2

    Plasmid

    #48146

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPK1E-1

    Plasmid

    #48145

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EC.SurA.pTYB1.a21-281&390-428.M231R

    Plasmid

    #15958

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Escherichia coli surA delta PPIase domain 2
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    residues 282-389 deleted; M231R mutation

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid

    #29656

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid

    #29659

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge