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We narrowed to 2,466 results for: crispr system

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Showing: 2201 - 2250 of 2466 results

  14. ADEPT-pCas9

    Plasmid
    #238032
    Purpose
    Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    spCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Promoter
    tet
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238032-plasmid-map-sequence-id-475985

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  22. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

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  25. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid
    #136140
    Purpose
    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    136140-plasmid-map-sequence-id-275306

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  32. ADEPT-pTarget-GFP

    Plasmid
    #238041
    Purpose
    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Promoter
    lac
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238041-plasmid-map-sequence-id-476072

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  33. ADEPT-pTarget-RFP

    Plasmid
    #238040
    Purpose
    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    mRFP1
    Use
    Synthetic Biology
    Promoter
    Lux
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238040-plasmid-map-sequence-id-476070

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  38. ADEPT-pTarget

    Plasmid
    #238033
    Purpose
    Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    ssrA
    Promoter
    J23119 (constitutive)
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238033-plasmid-map-sequence-id-471747

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  40. pLCHKOv3-mCherry-intergenic-As

    Plasmid
    #209037
    Purpose
    Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209037-plasmid-map-sequence-id-413702

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  41. pLCHKOv3-mClover-intergenic-As

    Plasmid
    #209036
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209036-plasmid-map-sequence-id-413695

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  42. pLCHKOv3-CD46 Ex3_1-Lb

    Plasmid
    #209027
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209027-plasmid-map-sequence-id-414806

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  43. pLCHKOv3-CD46 Ex3_2-Lb

    Plasmid
    #209028
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209028-plasmid-map-sequence-id-414792

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  44. pLCHKOv3-CD46 Ex3_3-Lb

    Plasmid
    #209029
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209029-plasmid-map-sequence-id-414782

    Enlarge

  45. pLCHKOv3-CD46 Ex3_1-As

    Plasmid
    #209031
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209031-plasmid-map-sequence-id-414800

    Enlarge

  46. pLCHKOv3-CD46 Ex3_2-As

    Plasmid
    #209032
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209032-plasmid-map-sequence-id-413524

    Enlarge

  47. pLCHKOv3-CD46 Ex3_3-As

    Plasmid
    #209033
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209033-plasmid-map-sequence-id-413572

    Enlarge

  48. pLCHKOv3-mClover-intergenic_2-As

    Plasmid
    #218814
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218814-plasmid-map-sequence-id-436107

    Enlarge

  49. pLCHKOv3-mClover-intergenic_3-As

    Plasmid
    #218815
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218815-plasmid-map-sequence-id-436108

    Enlarge

  50. pLCHKOv3-mClover-intergenic_4-As

    Plasmid
    #218816
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218816-plasmid-map-sequence-id-436109

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Showing: 2201 - 2250 of 2466 results