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pET28a-At-TRL

Plasmid

#32242

Depositor

Stan Fields, Jay Hesselberth

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pKI1.1

Plasmid

#85807

Purpose

CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDD2045

Plasmid

#101379

Purpose

Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Type

Empty backbone

Use

Unspecified

Availability

Academic Institutions and Nonprofits only

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHEE901

Plasmid

#91707

Purpose

CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Expression

Bacterial

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPIF6_X

Plasmid

#131182

Purpose

Expresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPIF6

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2+pAtUbq10

Plasmid

#170877

Purpose

Phytobrick - pAtUBQ10

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pAtUBQ10

Expression

Plant

Mutation

441C>T to remove BbsI restriction site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD+AtHSP18.2t

Plasmid

#170884

Purpose

Phytobrick - AtHSP18.2t

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

HSP18.2t

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CUC1 pBluescript

Plasmid

#12064

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 (CUC1 Mustard Weed)

Expression

Bacterial

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
CUC1 pGreenII0129

Plasmid

#12066

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCDFDuet-AtUBC8-S

Plasmid

#222713

Purpose

Expression AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBC8 (UBC8 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0022

Plasmid

#196147

Purpose

Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtARF18_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0012

Plasmid

#196146

Purpose

Gateway entry vector with Arabidopsis ANAC032 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNAC032_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0011

Plasmid

#196145

Purpose

Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtDREB26_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0002

Plasmid

#196142

Purpose

Gateway entry vector with Arabidopsis TGA2 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA2_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0009

Plasmid

#196143

Purpose

Gateway entry vector with Arabidopsis NLP6 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP6_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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