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pCAGGS-synPCB2.2

Plasmid

#139479

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG

Use

Tags

Expression

Mammalian

Mutation

V554L

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMM0649

Plasmid

#129010

Purpose

Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Use

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Use

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0655

Plasmid

#129016

Purpose

Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0654

Plasmid

#129015

Purpose

Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0653

Plasmid

#129014

Purpose

Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0650

Plasmid

#129011

Purpose

Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0648

Plasmid

#129009

Purpose

Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0651

Plasmid

#129012

Purpose

Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-clbH-C-A2-PCP S1524A

Plasmid

#114158

Purpose

Expresses ClbH-C-A2-PCP S1524A

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Use

Tags

S-Tag, Thrombin site

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH with S…

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 deltaC 350 (stop)

Plasmid

#67612

Purpose

bacterial expression of GST-PRMT9 missing residues 351-845

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Stop codons placed at aa 351 and 352 to create C …

Promoter

tac

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Scmh1-3'UTR

Plasmid

#61785

Purpose

Luciferase reporter containing the 3'UTR of mouse Scmh1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Scmh1 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR

Plasmid

#61772

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR

Plasmid

#61774

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Skp2-3'UTR

Plasmid

#61784

Purpose

Luciferase reporter containing the 3'UTR of mouse Skp2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Skp2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only