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C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-PLY-WT-charge+

Plasmid

#160538

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

W278K, L282K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 ER-ER (GB2666)

Plasmid

#160598

Purpose

two repeats of ER (Estradiol receptor)

Depositor

Diego Orzaez

Insert

ER-ER

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 fluc CDS

Plasmid

#153405

Purpose

Firefly luciferase coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

firefly luciferase CDS

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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S3_002_026_MV1

Plasmid

#134414

Purpose

pYTKMV1-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S3_003_026_096

Plasmid

#134406

Purpose

pYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pREV1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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S3_003_026_MV1

Plasmid

#134415

Purpose

pYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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S3_001_026_MV1

Plasmid

#134413

Purpose

pYTKMV1-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcF-2

Plasmid

#131025

Purpose

Single spacer array with spacer #2 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcF-3

Plasmid

#131024

Purpose

Single spacer array with spacer #3 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pWT029d

Plasmid

#96859

Purpose

(d)guanine _agRNA (GFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine _agRNA (GFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016h

Plasmid

#96854

Purpose

HHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016d

Plasmid

#96855

Purpose

dHHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029c

Plasmid

#96858

Purpose

guanine-agRNA (GFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016g

Plasmid

#96849

Purpose

processed HHR-sgRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

processed HHR-sgRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016f

Plasmid

#96850

Purpose

HHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016e

Plasmid

#96851

Purpose

dHHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016a

Plasmid

#96852

Purpose

HHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016b

Plasmid

#96853

Purpose

dHHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3766 HC_Kan_3'-LTR_p10

Plasmid

#100703

Purpose

part designed to occupy position 10 of EMMA. Functional category: Long Terminal Repeat

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Lentiviral 3' LTR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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