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Lenti-sgFlt4#2/Cre

Plasmid

#193216

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#2 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCol11a1#1/Cre

Plasmid

#193205

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col11a1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol11a1#1 (Col11a1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCol11a1#2/Cre

Plasmid

#193206

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col11a1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol11a1#2 (Col11a1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgApc#2/Cre

Plasmid

#193202

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Apc gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgApc#2 (Apc Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFlt4#1/Cre

Plasmid

#193215

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#1 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgGrin2a#1/Cre

Plasmid

#193217

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Grin2a gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgGrin2a#1 (Grin2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFam135b#1/Cre

Plasmid

#193213

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP11

Plasmid

#180344

Purpose

mammalian expression of human SEPT9_i1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP10

Plasmid

#180345

Purpose

mammalian expression of human SEPT9_i3 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP11

Plasmid

#180346

Purpose

mammalian expression of human SEPT9_i3 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-cPHx1

Plasmid

#183674

Purpose

PtdIns(3,4)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 169-329

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-FLAG-CBPΔN

Plasmid

#182840

Purpose

A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)

Tags

FLAG tag

Expression

Insect

Promoter

MT

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-hPE(-)-Luc

Plasmid

#172004

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-mPE(-)-Luc

Plasmid

#172006

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-mPE(+)-Luc

Plasmid

#172005

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ285-RVR

Plasmid

#138121

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N512R, K518V and N522R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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61427-sgHtt_10

Plasmid

#157861

Purpose

Expresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM system

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Htt (Htt Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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61427-sgHtt_6

Plasmid

#157860

Purpose

Expresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM system

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Htt (Htt Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge